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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Radiosensitivity of Vetiver to Acute and Chronic Gamma Irradiation) ผู้เขียน: ดร.นวลฉวี รุ่งธนเกียรติ, ศาสตราจารย์ ,  Peranut Jompuk, Titirat Rattanawongwiboon, Ratchanewan Puingam สื่อสิ่งพิมพ์:pdf AbstractNuclear technology has been widely applied in agriculture for crop improvement. In this study, the radiosensitivities of native Thai vetiver, Chrysopogon zizaninoides, the Kamphaeng Phet 2 and the Surat Thani ecotypes, and Chrysopogon nemoralis, the Ratchaburi ecotype, were investigated with acute and chronic gamma irradiation. Vetiver tillers of the Kamphaeng Phet 2 and the Surat Thani ecotypes were exposed to acute irradiation with gamma radiation from Cs-137 at doses of 0, 10, 20, 30, 40, 50, 60, 80 and 100 Gy. For chronic irradiation with a Co-60 source, the Kamphaeng Phet 2 tillers were exposed to 0, 65, 104, 116, 157, 182 and 205 Gy whereas the Ratchaburi tillers were exposed to 0, 63, 87, 127, 150, 173 and 213 Gy. The survival rate and growth performance measured by plant height, shoot and root dry weight of vetiver at 90 d after irradiation were recorded for median lethal dose (LD50/90) and 50% growth reduction dose (GR50/90) determination. Gamma radiosensitivity differences were observed between the irradiation methods and between the vetiver ecotypes. Acute irradiation caused higher radiosensitivity of vetiver than chronic irradiation. The LD50/90 values of the Kamphaeng Phet 2 ecotype to acute and chronic irradiation were 82 and 100 Gy, respectively. In general, the survival rate and growth of vetiver decreased with an increase in the gamma radiation doses. However, chronic irradiation of the Kamphaeng Phet 2 ecotype at 65 Gy gave higher shoot and root dry weights than the control treatment (0 Gy). The GR50/90 values of the Kamphaeng Phet 2 and the Surat Thani ecotypes to acute irradiation were 48 and 75 Gy for plant height and were 30 and 43 Gy for total dry weight, respectively. For chronic irradiation, the GR50/90 values of the Kamphaeng Phet 2 and the Ratchaburi ecotypes were 118 and 109 Gy for plant height and 121 and 67 Gy for total dry weight, respectively. This information will be useful for radiation-induced mutations in vetiver research. |
 ที่มา:มหาวิทยาลัยเกษตรศาสตร์หัวเรื่อง:การปรับปรุงพันธุ์ไม้น้ำสกุล Anubias โดยการเพาะเลี้ยงเนื้อเยื่อร่วมกับการฉายรังสีแกมมาแบบเฉียบพลันและแบบเรื้อรัง |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Acute and Chronic Gamma Irradiations on In vitro Culture of Anubias congensis N.E. Brown) ผู้เขียน:ปกรณ์ ตั้งปอง, Thanya Taychasinpitak, ดร.ชูศักดิ์ จอมพุก, รองศาสตราจารย์ , ดร.พีรนุช จอมพุก, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractPlantlets of Anubias congensis N.E. Brown cultured on MS medium were irradiated with gamma rays for both acute and chronic treatments of 0, 20, 40, 60, 80 and 100 Gy at a dose rate of 264 Gy/hr (acute) and 0, 14.34, 28.60, 31.24, 42.90, 51.16, 65.55, 82.42, 91.69, 105.99 and 120.30 Gy at a dose rate of 0.71 Gy/hr (chronic). The calculated LD50(60) for the acute irradiation was 32.12 Gy. It was not possible to calculate the LD50(60) for the chronic irradiation because the survival rate was 100% for all treatments. Following acute gamma irradiation, the number of roots, number of shoots, number of leaves, length and width of leaves and length of roots in the M1V1 generation of A. congensis N.E. Brown plantlets were less than the control. With chronic gamma irradiation, there was no difference in most parameters with the exception of the 105.99 and 120.30 Gy treatments, in which the number of roots was greater than the control. Some abnormalities observed in the M1V1 generation exposed to acute irradiation that were genetically transferred to the M1V3 generation included narrow leaves, undulate leaf margins, and dwarfism with light green leaves. The mutation frequencies for these characters were 11.67, 26.67 and 6.67% for a dose of 20, 40 and 60 Gy, respectively. For the samples exposed to chronic irradiation, mutations observed in the M1V3 generation were dwarfism and albinism. The mutation frequencies were 1.67, 1.67, 3.33, 5.00, 11.67 and 10.00% at a treatment dose of 14.34, 28.60, 31.24, 91.69, 105.99 and 120.30 Gy, respectively. No mutation could be detected in the M1V3 generation irradiation with chronic gamma rays at 42.90, 51.16, 65.55 and 82.42 Gy. The potential mutants of A. congensis N.E. Brown in this research were the undulate leaf margins and dwarfism with light green leaves. All the mutation characters were maintained through tissue culture. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Acute and Chronic Gamma Irradiation on Tissue Culture of Cryptocoryne wendtii “brown”) ผู้เขียน:ดร.พีรนุช จอมพุก, รองศาสตราจารย์, ดร.ชูศักดิ์ จอมพุก, รองศาสตราจารย์, Apira Bunjongpetch, ปกรณ์ ตั้งปอง สื่อสิ่งพิมพ์:pdf AbstractThe value of aquatic plant exports from Thailand is rising and there is a demand for interesting new varieties of aquatic plants. The objective of this research was to find the appropriate dose of gamma irradiation to induce mutations in Cryptocoryne wendtii “brown.” C. wendtii “brown” was cultured aseptically on MS medium supplemented with BA (2mg/l) and NAA (0.25 mg/l). Samples of the tissue- cultured plantlets were exposed to acute gamma radiation using a Mark I Gamma Irradiator with a Cesium-137 source at doses of 0, 10, 20, 30, 40 and 50 Gy. Other samples were exposed to chronic gamma radiation in a gamma room using a Cobalt-60 source at the Gamma Irradiation Service and Nuclear Technology Research Center of the Faculty of Science, Kasetsart University. The samples were placed at 1.5 and 2.5 m from the source with a dose rate of 1.614 and 0.537 Gy/hr, respectively. The samples at 1.5 m received a total dose of 238.87 and 477.74 Gy (0 for the control) and those at 2.5 m received a total dose of 10.75, 21.50, 32.24 and 42.99 Gy (0 for the control). The number of dead and surviving plantlets and the number of new shoots were recorded at 45 days after irradiation and compared with the controls to calculate the LD50(45) and GR50(45). Under acute irradiation, the LD50(45) and GR50(45) for C. wendtii “brown” were found to be 23 and 20 Gy, respectively. Some variations were observed, such as clumping, dwarfism, darker brown leaves, furled leaves and narrow leaves. Under chronic irradiation it was not possible to calculate the LD50(45) or GR50(45) because at 1.5 m from the source all the samples died and at 2.5 m from the source more than 50% of the samples survived. Some variations were observed, such as shorter plants, green leaves, increased branching, shorter petioles and longer, narrower leaves of a greenish-brown color. |
ที่มา:มหาวิทยาลัยเกษตรศาสตร์หัวเรื่อง:การปรับปรุงพันธุ์ไม้น้ำสกุล Anubias โดยการเพาะเลี้ยงเนื้อเยื่อร่วมกับการฉายรังสีแกมมาแบบเฉียบพลันและแบบเรื้อรัง |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Chronic Gamma Irradiation on Adventitious Plantlet Formation of Saintpaulia ionantha (African Violet) Detached Leaves) ผู้เขียน:นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractFormation of adventitious plantlets on unrootedly detached leaves of two African violet (Saintpaulia ionantha) cultivars, pink and violet flowers, chronically gamma-irradiated in gamma room at The Gamma Irradiation Service and Nuclear Technology Research Center, Kasetsart University was compared. Detached leaves were immediately planted after detachment in plastic trays containing peat moss, 18 leaves per treatment with 3 replications. Three dose rates (rad/h) with 3 doses (rad)/dose rate, were applied to the irradiated samples while the controls were placed outside the gamma room. Three months after irradiation, the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf were recorded. After that, the young plantlets were transferred to the new pots for further observation on plant growth and mutation characters. The results revealed that the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf varied slightly with radiation doses but were not significantly different at different dose rates. Radiosensitivity was noticed to be higher in pink flower cultivar than the violet one. M1V1 plantlets will be followed up for growth and mutation character observations. |
ที่มา:มหาวิทยาลัยเกษตรศาสตร์หัวเรื่อง:การปรับปรุงพันธุ์ไม้น้ำสกุล Anubias โดยการเพาะเลี้ยงเนื้อเยื่อร่วมกับการฉายรังสีแกมมาแบบเฉียบพลันและแบบเรื้อรัง |
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ที่มา:Agricultural Science Journal (วารสารวิทยาศาสตร์เกษตร)หัวเรื่อง:ผลของการฉายรังสีแกมมาแบบเฉียบพลันและแบบโครนิกต่อต้นอ่อนที่ได้จากการเพาะเลี้ยงเนื้อเยื่อดาหลา |